Well first of all you should tell us what the age of the seedlings; then the species, and after that the location of them.
I wouldn't repot, slip pot, or up pot a seedling that's doing well and is not root bound.
But if you must repot it, the answer is...
It depends.
Many species can be barerooted at this stage, so in theory you can change all the soil for the new one.
But conifers in general like to keep some of the old soil, so I would mix the two.
But... Suggestions with no data have no value I guess...